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. 2005 Aug;79(16):10200–10209. doi: 10.1128/JVI.79.16.10200-10209.2005

FIG. 1.

FIG. 1.

Expression of HIVMN envelope by replicating and nonreplicating Ad recombinants. The env gene expression of replicating and nonreplicating Ad-HIVMNenv/rev recombinants was analyzed in 293 or 293-ORF6 cells and in A549 cells by Western blotting with the HIV-1 gp41 monoclonal antibody 2F5. Equivalent PFU of each recombinant were used to infect the indicated cell lines. The nonreplicating Ad7-ΔE1ΔE3-HIV-1MNenv/rev recombinant did not grow in 293 cells but required additional complementation by ORF6 of the Ad5 E4 gene present in 293-ORF6 cells (5) as described previously (29).