TABLE 1.
Resistance profile of infectious clones
| Clone | Genotype | Fold reduction of susceptibilitya
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RTV | SQV | APV | NFV | IDV | LPV | NVP | EFV | DLV | ABC | ADV | ddI | 3TC | d4T | TFV | ddC | ZDV | ||
| 1035d1-3 | T12S K43R L63P | 0.5 | 0.4 | 0.5 | 0.6 | 0.5 | 0.5 | 1.1 | 0.5 | 1.5 | 0.6 | 0.7 | 0.8 | 0.7 | 0.8 | 0.8 | 0.9 | 0.7 |
| 1035m6-5 | T12S K43R M46I L63P V82F | 14.8 | 0.2 | 2.2 | 7.4 | 2.3 | 4.5 | 0.2 | 0.3 | 0.6 | 0.5 | 0.7 | 0.8 | 1.0 | 0.8 | 0.7 | 0.9 | 0.5 |
| 1035m12-10 | T12S K43R M46I I54V Q61H L63P V82F | 30.8 | 0.1 | 1.7 | 19.2 | nd | 5.8 | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd | nd |
| 1118d1-15 | N37S L63P V77I I93L | 1.2 | 0.9 | 1.0 | 1.9 | 1.4 | 0.9 | 0.6 | 0.6 | 1.6 | 0.8 | 0.8 | 0.9 | 0.9 | 1.0 | 0.9 | 1.0 | 0.7 |
| 1118m5-1 | N37S I54V L63P V77I V82T I93L | 6.5 | 0.6 | nd | 2.3 | 2.3 | 3.3 | 0.6 | 0.4 | 0.6 | 0.7 | 0.8 | 1.0 | 0.6 | 1.1 | 0.6 | 0.8 | 0.4 |
| 1118m12-6 | L33F E34Q N37S I54V Q58E L63P A71V V771 V82A L90M I93L | 59.5 | 1.8 | 1.6 | 9.0 | 4.1 | 2.9 | 0.4 | 0.4 | 1.0 | 0.7 | 0.8 | 0.8 | 0.9 | 0.9 | 0.8 | 1.0 | 0.6 |
| 1137d1-12 | N37E D60E I64V | 1.2 | 1.0 | 1.1 | 1.6 | 1.1 | 1.0 | 0.7 | 0.7 | 1.8 | 0.8 | 0.8 | 0.9 | 0.9 | 0.9 | 0.8 | 0.9 | 0.7 |
| 1137M4-9 | N37E I54V D60E 164V V82A | 3.4 | 0.4 | 0.3 | 0.5 | 0.6 | 1.2 | 0.4 | 0.4 | 0.4 | 0.6 | 1.1 | 0.7 | 1.8 | 1.5 | 0.9 | 0.8 | 0.6 |
| 1137m4-13 | N37E F53I D60E I64V V82A | 7.7 | 0.7 | 1.1 | 1.8 | 1.6 | 1.7 | 0.4 | 0.4 | 1.0 | 0.6 | 0.6 | 0.8 | 0.8 | 0.8 | 0.7 | 0.9 | 0.5 |
| 1137m12-6 | L24I N37E M46I I54V D60E L63P I64V V82A | 33.9 | 1.3 | 1.6 | 6.7 | 6.9 | 13.6 | 0.2 | 0.4 | 0.7 | 0.6 | 0.7 | 0.8 | 0.7 | 0.8 | 0.8 | 0.8 | 0.5 |
| wtb | N37S | 0.6 | 0.6 | 0.6 | 0.5 | 0.5 | 0.6 | 0.5 | 0.6 | 1.4 | 0.8 | 0.8 | 0.8 | 0.9 | 0.9 | 0.8 | 0.9 | 0.6 |
a
Relative to NL4-3. RTV, ritonavir; SQV, saquinavir; APV, amprenavir; NFV, nelfinavir; IDV, indinavir; LPV, lopinavir; NPV, nevirapine; EFV, efavirenz; DLV, delavirdine; ABC, abacavir; ADV, adefovir; ddI, didanosine; 3TC, lamivudine; d4T, stavudine; TFV, tenofovir; ddC, zalcitabine; ZDV, zidovudine. Underlined numbering indicates greater than 2.5-fold deviation from control before rounding; italics indicates low luciferase activity resulting in less reliable measurements. nd, not determined (low luciferase activity).
b
Parental strain for generation of infectious clones.