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. 2005 Aug;79(16):10776–10787. doi: 10.1128/JVI.79.16.10776-10787.2005

FIG. 6.

FIG. 6.

Effect of okadaic acid on A-MLV and VSV infection. (A) NIH 3T3 cells were treated with 0.1 μM okadaic acid (OA) for 30 min. Subsequently, the cells were infected with A-MLV or VSV pseudotyped vectors for 4 and 6 h in the presence of okadaic acid. Noninternalized viruses were inactivated using citrate buffer and 48 h after vector addition, infected β-galactosidase-positive cells were counted. The numbers of infected cells are normalized to the mock values. Shown are the means of three (A-MLV) or two (VSV) independent experiments done in duplicate. (B) NIH 3T3 cells were incubated with A-MLV or VSV pseudotyped vectors for 4 or 6 h, washed, and treated with 0.1 μM okadaic acid for 30 min. Subsequently, noninternalized viruses were inactivated using citrate buffer and 48 h after vector addition, infected β-galactosidase-positive cells were counted. The numbers of infected cells are normalized to the mock values. Shown are the means of two independent experiments done in duplicate.