FIG. 8.

Colocalization of fusion-defective Gag-YFP A-MLV particles with caveolin-1 in NIH 3T3 cells. NIH 3T3 cells were incubated for 6 h with Gag-YFP A-MLV particles (green), which are fusion defective due to an unprocessed envelope protein. The cells were permeabilized, fixed with paraformaldehyde, and stained for caveolin-1 (red). The cells were investigated using three-dimensional scanning confocal microscopy. Shown is one example of a YZ section showing colocalization of Gag-YFP A-MLV with caveolin-1. Arrows: Gag-YFP A-MLV particles colocalized with caveolin-1. Arrowhead: intracellular Gag-YFP A-MLV particle colocalized with caveolin-1.