FIG. 10.

Effect of ERK inhibitor U0126 on host gene expression. (A) Semiquantitative RT-PCR confirmation of DUSP5 gene expression in U0126-treated and untreated KSHV-infected HFF cells. DNase-treated total RNAs were subjected to RT-PCR using specific primers. Successive samples were removed from every three cycles (14 to 41) and resolved on agarose gel, and the change in DUSP5 expression (n-fold) was calculated after normalizing to the β-actin gene. Ethidium bromide-stained RT-PCR amplified DUSP5 gene products after gel electrophoresis are shown. Representative amplification of the β-actin gene and reactions of DNA-PCR (-RT) are shown. (B) Quantitation of differential amplification of KSHV-infected and U0126-treated HFF cell RNA samples. IDVs corresponding to the sum of pixel intensities after background corrections were recorded for both the KSHV-infected and U0126-pretreated samples at linear points on the amplification curve and changes expression were created after normalizing to the β-actin gene. Representative graph depicts the change in DUSP5 expression (n-fold) as calculated from the change in the IDV. (C) Histogram representing the percent inhibition of host genes induced by KSHV infection in the presence of U0126. Expression of the specific gene by KSHV at indicated time points was considered 100%.