FIG. 3.
EMCV induces MAP kinase, CREB, and NF-κB activation in PKR+/+ and PKR−/− macrophages. Macrophages (4 × 105/400 μl cCMRL-1066) isolated from PKR+/+ (A) or PKR−/− (B) mice by peritoneal lavage were treated with EMCV (1 PFU/cell) at 37°C for the indicated times, and JNK, p38, ERK, and CREB activation was determined by Western blot analysis using phospho-specific antisera. EMCV-induced NF-κB activation was evaluated by determining the loss of IκBα by Western blot analysis. The levels of GAPDH were determined as a protein loading control. The results are representative of three independent experiments.