FIG. 4.

EMCV-induced PKR activation in RAW264.7 cells and primary macrophages. RAW264.7 cells (A) or peritoneal macrophages (B to E) were treated with EMCV (1 PFU/cell) at 37°C for the indicated times. The cells were isolated, and PKR activation was determined by a Western blot analysis of PKR phosphorylation (A and B) or eIF2α phosphorylation (C to E) using phospho-specific antisera. The total levels of PKR or eIF2α were determined by Western blot analysis to control for protein loading. The results are representative of two or three independent experiments.