FIG. 5.
IFN-α/β response is not required for EMCV-induced iNOS or IL-1β expression by macrophages. Macrophages (4 × 105/400 μl cCMRL-1066) isolated from wild-type (IFN-α/βR+/+) mice or mice deficient in the IFN-α/βR (IFN-α/βR−/−) were infected with EMCV (1 PFU/cell) in the presence and absence of IFN-γ (150 U/ml) for 24 h, and nitrite production (A) and IL-1 release (C) were determined in cell culture supernatants by a Greiss assay and a RINm5F cell bioassay, respectively. The expression of iNOS (B) and IL-1β (D) following a 24-h treatment with EMCV in the presence or absence of IFN-γ was determined in cell lysates by Western blot analysis. The results for nitrite production and IL-1 release are means ± SEM for three independent experiments, and the results for iNOS and IL-1β expression are representative of three independent experiments.