FIG. 5.

Real-time PCR suggests that the ORF17 RNA is specifically incorporated into KSHV virions. RNA from induced BCBL-1 cells (3 days postinoculation) was reverse transcribed with an oligo(dT) primer and subjected to real-time PCR using gene-specific primers. Standard curves were generated for each primer/probe set (listed in Table 2) and then used to calculate the relative amounts of transcripts present in the sample. Levels of KSHV transcripts were normalized to levels of GAPDH transcript. The graph displays the normalized levels of each transcript (n-fold) over GAPDH. The data values are listed with the corresponding bars, and the error bars representing the standard deviations are shown.