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. 2005 Aug 2;33(14):4395–4403. doi: 10.1093/nar/gki741

Figure 8.

Figure 8

ITC measurement of the interaction of K+ ions with TBA in 10 mM Cs–HEPES, pH 7.5 at 20°C. (A) The upper curve represents heats of KCl dilution in the same buffer. The lower curve shows the heat released upon titration of K+ into a 10 μM TBA solution by injection of various volumes (2, 4, 8 and 16 μl) of 7 mM KCl solution. (B) Plot showing the cumulative heats of reaction as a function of [K+]/[TBA], based on the data shown in A. The data are fit to a model that assumes a single non-cooperative binding site (45).