Fig. 1.

Expression of cytokeratins in model human epithelial cell lines and effects of transfection with Nox1. a Phase contrast pictures showed morphology of the parental GM16 cells in keratinocyte growth medium (KGM) and progenitor NuB2 and NuB1 cells in DMEM. b Expression of CK18 was higher in spindle NuB1 cells compared with cobblestone cell lines including FuB1, NuB2 as well as GM16 cells, which were cultured in KGM [GM16 (KGM)] or adapted to grow in DMEM [GM16 (DMEM)]. c Transfection of GM16 cells with Nox1 plasmid increased CK18 but not CK8 protein in KGM. d Plasmid containing full-length Nox1, but not truncated version of Nox1 (Nox1-tv), increased CK18 protein expression in GM16 cells cultured in KGM