Figure 5:
Up-regulation of PTEN inhibited PLK1. (a and b) Effect of PTEN overexpression on PLK1 protein expression. (c-e) Western blot verified the efficiency of overexpressions of PLK1 and PTEN. (f and g) RT-qPCR verified the efficiency of overexpressions of PLK1 and PTEN. n = 3 ✶✶P < 0.01, ✶✶✶ P < 0.001. Ov-PLK1: PLK1 overexpression plasmid, Ov-PLK1+Ov-NC: negative control to overexpression plasmid, Ov-PLK1+ Ov-PTEN: PTEN and PLK1 overexpression plasmid, RT-qPCR: Real-time quantitative polymerase chain reaction.
