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Primer sequences used for Real-Time PCR for the mice genes (from the Origene company website)
| Target templates | Primer | Sequence (5'->3') |
|---|---|---|
| beta-actin | Forward | CATTGCTGACAGGATGCAGAAGG |
| Reverse | TGCTGGAAGGTGGACAGTGAGG | |
| IL-1β | Forward | TGGACCTTCCAGGATGAGGACA |
| Reverse | GTTCATCTCGGAGCCTGTAGTG | |
| IL-6 | Forward | TACCACTTCACAAGTCGGAGGC |
| Reverse | CTGCAAGTGCATCATCGTTGTTC | |
| TNF-α | Forward | GGTGCCTATGTCTCAGCCTCTT |
| Reverse | GCCATAGAACTGATGAGAGGGAG |
