Table 1.

Primers used for plasmid construction

Gene name	Forward primer sequence (5ʹ–3ʹ)	Reverse primer sequence (5ʹ–3ʹ)
HY12-2C HY12-VP1 HY12-VP2 HY12-VP3 HY12-VP4 HY12-2A HY12-2B HY12-3AB HY12-3C HY12-3D 2C (aa 1–125) 2C (aa 126–253)	CCGGAATTCTCAGACAATTGGAT CCGGAATTCAATGACCCAGGGAA CCGGAATTCTCTCCGTCAGCAGAA CCGGAATTCGGCCTTCCAACAAA CCGGAATTCATGGGCGGTCAGTT CCGGAATTCGGGCCTTTTGGACA CCGGAATTCGGCATTACTGATTAT CCGGAATTCGGCCCCGTAACCTA CCCTCGAGGGACCACTCTTTGA CCGGAATTCGGGCAAATAGAATAC GCGTCGACTCAGACAATTGGATAA GCGTCGACCTCATACATGGCTCTCC	ATTTGCGGCCGCTTATTGAAAAAGG ATTTGCGGCCGCGTACGAGGTGAG ATTTGCGGCCGCTTGGTTTGATGCAA ATTTGCGGCCGCCTGTAGTGCGGCTG ATTTGCGGCCGCTTTCAGCGGAACG ATTTGCGGCCGCTTGCTCCATAGCGT ATTTGCGGCCGCTTGCCTCTCTGCCA ATTTGCGGCCGCTTGAGTTTGAACCT ATTTGCGGCCGCTTGTTCAACAGTAA ATTTGCGGCCGCTTAGAAAGAATCT CGGGATCCTTATGCACATACGGGTTC CGGGATCCTTATGTCTTTCTGTACT
2C (aa 254–329) 2C (aa 1–121) 2C (aa 1–117) 2C (aa 1–113) p65 p65 (aa 1–290) p65 (aa 291–551) p65 (aa 1–194) p65 (aa 195–290) IKBKB	GCGTCGACTATAAGAAGAATGGAT GCGTCGACTCAGACAATTGGATAA GCGTCGACTCAGACAATTGGATAA GCGTCGACTCAGACAATTGGATAA CGCGGATCCATGGACGAACTGTTCC CGCGGATCCATGGACGAACTGTTCC CGGGATCCATGGATACAGACGATC CGCGGATCCATGGACGAACTGTTCC CGCGGATCCATAAGATCTGCCGAG CCAAGCTTATGAGAAGGCTGACCC	CGGGATCCTTATTGAAAAAGAGCCTC CGGGATCCTTATTCAATGCGATTC CGGGATCCTTACTTGGTCTTGAACTG CGGGATCCTTACTGCATGGCACCGAG TGCTCTAGAGGAGCTGATCTGACT TGCTCTAGATGGCAGGTACTGGAATT TGCTCTAGAGCTGATCTGACTCAGC TGCTCTAGAGAGCTCGGCAGTGTT TGCTCTAGATGGCAGGTACTGGAATT GCTCTAGATGAGGCCTGCTCCAGG

The restriction enzyme cutting sites are highlighted in bold.