Table 2.
Chemicals, peptides, and recombinant proteins.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Red blood cell (RBC) lysis buffer, 1x | ThermoFisher Scientific | Cat. no. 00-4333-57 |
| RIPA buffer | Alfa Aesar | Cat. no. J63306 |
| Fetal bovine serum (FBS) | Genesee Scientific | Cat. no. 25-550H |
| Collagenase | Sigma-Aldrich | Cat. no. C6885 |
| Roswell Park Memorial Institute (RPMI)- 1640 medium | Corning | Cat. no. 10-041-CV |
| Dulbecco’s modified Eagle medium (DMEM) | Corning | Cat. no. 10-013-CV |
| Ammonium-chloride-potassium (ACK) Lysing Buffer | ThermoFisher Scientific | Cat. no. A1049201 |
| Paraformaldehyde, 4% | Alfa Aeser | Cat. no. J61899 |
| BCA protein assay kit | ThermoFisher Scientific | Cat. no. 23225 |
| Laemmli sample buffer, 4x | Bio-Rad | Cat. no. 1610747 |
| 2-β-mercaptoethanol | Alfa Aesar | Cat. no. A15890 |
| Tris-buffered saline (TBS) containing 0.2% Tween 20 | ThermoFisher Scientific | Cat. no. 28360 |
| Polyvinylidene fluoride (PVDF) membranes | Bio-Rad | Cat. no. 1620174 |
| Intercept blocking buffer | LI-COR Biosciences | Cat. no. 927-60001 |
| Qiazol | Qiagen | Cat. no. 79306 |
| Lipopolysaccharide | Sigma | Cat. No. L4391 |
| Recombinant Mouse M-CSF | BioLegend | Cat. No. 576406 |
| Recombinant Mouse IFN-γ | BioLegend | Cat. No. 575306 |
| Penicillin-Streptomycin | Gibco | Cat. No. 15140-122 |