Figure 4.

Effects of anisomycin (ANI) on olfactory recognition memory performance of adult male mice as tested in the social discrimination procedure. ANI was injected subcutaneously (ANI; 150 mg/kg b. wt.; ▾) at different time points related to sampling on juvenile discrimination at an RI of 24 h. ANI interfered with juvenile discrimination when applied 20 min before (A; left and middle panels), immediately after (0 h; B right panel), and 6 (D; right panel) but not 3 h or 18 h (E; right panel), after (C; right panel) sampling. For control sessions animals received the same volume of sterile NaCl solution (0.9 %; NaCl, ▿; A–E, left panels). To control for nonspecific effects of ANI treatment, an additional control session was performed for A. For each animal, investigation during sampling was allowed for exactly the same duration taken under ANI treatment. This restriction did not affect the juvenile discrimination abilities (right panel). ^**P < 0.01, ^***P < 0.001, paired Student's t-test.