FIG. 4.

Kinetics of 3,4-DMC formation. Cells expressing PH were used at a constant concentration (0.5 mU/ml), and the rate of 3,4-DMC formation was measured as a function of the concentration of cells expressing ToMO (▪) or (E103G)-ToMO (□) in the presence of 40 μM o-xylene. The rate of 3,4-DMC production was measured by the continuous coupled assay with C2,3O as described in Materials and Methods. The inset shows linearization of the data obtained by plotting rates (μM min⁻¹) as a function of the rate/[ToMO] ratio (μM min⁻¹/mU ml⁻¹). wt, wild type.