. 2005 Aug;71(8):4744–4750. doi: 10.1128/AEM.71.8.4744-4750.2005

TABLE 2.

Apparent kinetic constants of ToMO and ToMO mutants for phenol and cresols

Compound	Parameter	ToMO	(E103G)-ToMO	(E103L)-ToMO	(E103M)-ToMO
Phenol	K_m(μM)^a	2.18	2.7	15	2.5
	k_cat(s⁻¹)^b	1.0	1.0	0.89	0.29
	k_cat/K_m(s⁻¹μM⁻¹)	0.46	0.37	0.064	0.1
o-Cresol	K_m(μM)^a	6	11.2
	k_cat(s⁻¹)^b	0.9	1.5
	k_cat/K_m(s⁻¹μM⁻¹)	0.15	0.13
m-Cresol	K_m(μM)^a	9.38	13.2
	k_cat(s⁻¹)^b	0.44	1
	k_cat/K_m(s⁻¹μM⁻¹)	0.047	0.076
p-Cresol	K_m(μM)^a	13.3	11
	k_cat(s⁻¹)^b	0.63	0.59
	k_cat/K_m(s⁻¹μM⁻¹)	0.047	0.054

The errors for K_m values were less than 10%.

The errors for k_cat values were about 26%. The errors for the k_cat values for phenol were about 20%.