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Regioselectivities of PH and ToMO (wild type and mutants) for toluene, m-cresol, o-xylene, and 3,4-DMP
| Substrate | Product | % witha:
|
|||
|---|---|---|---|---|---|
| ToMO | (E103G)-ToMO | (E103L)-ToMO | (E103M)-ToMO | ||
| Toluene | o-Cresol | 36 | 9 | 20 | 47 |
| m-Cresol | 19 | 6 | 11 | 19 | |
| p-Cresol | 45 | 85 | 69 | 34 | |
| o-Xylene | 2,3-DMP | 20 | 1 | 6 | 18 |
| 3,4-DMP | 80 | 99 | 94 | 82 | |
| m-Cresol | 3-MC | 5 | 2 | NDb | ND |
| 4-MC | 95 | 98 | ND | ND | |
| 3,4-DMP | 3,4-DMC | 10 | 1 | ND | ND |
| 4,5-DMC | 90 | 99 | ND | ND | |
The errors were ∼1%.
ND, not determined.
