TABLE 4.
Regioselectivities of PH/ToMO (wild type and mutant) mixtures on toluene
| Product | % witha:
|
|||
|---|---|---|---|---|
| Wild-type ToMO/PH | (E103G)-ToMO/PH | (E103L)-ToMO/PH | (E103M)-ToMO/PH | |
| 3-MC | 55 (54)b | 14 (14) | 29 (30) | 67 (65) |
| 4-MC | 45 (46) | 86 (86) | 71 (70) | 33 (35) |
a
The errors were ∼1.5%.
b
The values in parentheses are theoretical percentages of 3- and 4-MC calculated by assuming that ToMO (wild type and mutants) converts toluene to cresols and PH converts cresol isomers to MC. Theoretical values were calculated with the following equation: 3-MC% = o-C% + 0.96 × m-C%, where 3-MC% is the percentage of 3-MC compared with the total amount of methylcatechols produced and o-C% and m-C% are the percentages of o-cresol and m-cresol, respectively, compared with the total amount of cresols produced by ToMO. For details see the accompanying paper (4).