FIG. 1.

(a) Growth inhibition assays after 16 h of growth in 0 to 4.5 mM CoCl₂ in the absence of nisin. Symbols: ▴, NZ9800(pNZ8048); ○, NZ9800(pSM04); •, NZ9800(pSM02). (b) Overexpression of corA. A growth inhibition assay of NZ9800(pSM02) after 16 h of growth in 0 to 4.5 mM CoCl₂ in the presence of 0 ng of nisin/ml (•), 5 ng of nisin/ml (□), and 50 ng of nisin/ml (▴). For both graphs, construction schemes for the vectors are shown. The vector pNZ8048 is represented as a thick gray bar, the nisin inducible promoter is shown as a small thin black arrow, orf18 is represented as a light gray boxed arrow, and corA is represented as a dark gray boxed arrow.