TABLE 1.

Kinetic properties of AroA_{E. coli}, AroA_{E. coli}-G96A, and AroA_{P. putida}^a

Enzyme	Sp actb (nkat/mg protein)	Km[PEP]c (μM)	Km[S3P]d (μM)	Ki[glyphosate]f (mM)
AroAE. coli	7.43 ± 0.12	9.43 ± 1.98	27.45 ± 5.73	(0.608 ± 0.151) × 10−3
AroAE. coli-G96A	1.25 ± 0.04	234.86 ± 7.79	NDe	2.525 ± 1.062
AroAP. putida	9.36 ± 0.27	10.36 ± 4.12	19.50 ± 1.18	0.183 ± 0.084

^aThe results presented are averages of two sets of experiments done in triplicate.

^bDetermined at 1.0 mM PEP and 1.0 mM S3P.

^cDetermined by the Lineweaver-Burk method. The PEP concentration was varied from 20 to 400 μM, while the S3P concentration was fixed at 1 mM.

^dDetermined by the Lineweaver-Burk method. The S3P concentration was varied from 20 to 250 μM, while the PEP concentration was fixed at 1 mM.

^eND, not determined.

^fCompetitive inhibition by glyphosate with respect to PEP was demonstrated by lines converging on the x axes of Lineweaver-Burk plots.