TABLE 1.
E. coli and M. acetivorans plasmid constructs
| Plasmid | Parent | Characteristicsa | Source or reference |
|---|---|---|---|
| pCR2.1 | Cloning vector, Ampr, lacZα | Invitrogen | |
| pBAD-TOPO | Cloning vector, Ampr, lacZ | Invitrogen | |
| pWM307 | Ori R6K, Ori pC2A, Ampr, Purr | 9 | |
| pWM315 | Ori R6K, Ori pC2A, Ampr, Purr, lacZα | 9 | |
| pCDH1 | pUC18 | cdhA,′ Ampr | 15 |
| pcdhP-lacZ | pBAD-TOPO | cdhA′::lacZ; Ampr, Ori pBR322 | This study |
| pKJ104 | pWM315 | cdhA′::lacZ, Ori pC2A, Ampr, Purr, Ori R6K | This study |
| pEA103 | pWM307 | cdhA′::lacZ, Ori pC2A, Ampr, Purr, Ori R6K | This study |
| pCDHΔ64 | pKJ104 | cdhA′Δpromoter::lacZ, Ori pC2A, Ampr, Purr, Ori R6K | This study |
| pEA110 | pEA103 | cdhA′Δpromoter::lacZ, Ori pC2A, Ampr, Purr, Ori R6K | This study |
a
cdhA′, cdh promoter; cdhA′ Δpromoter::lacZ, promoterless vector.