TABLE 2.
Effect of substrates on cdh promoter-lacZ expression
| Vector(s) | Substrate | Specific activity (mean ± SD)a |
|---|---|---|
| pEA103 | Sodium acetate | 23.77 ± 1.62 |
| pEA103 | Methanol | 0.44 ± 0.09 |
| pEA103 | Trimethylamine | 0.77 ± 0.51 |
| pEA103 | Monomethylamine | 0.66 ± 0.06 |
| pKJ104 | Sodium acetate | 24.22 ± 1.25 |
| pKJ104 | Methanol | 0.95 ± 0.20 |
| pKJ104 | Trimethylamine | <0.1 |
| pEA110, pCDHΔ64 | Sodium acetate | <0.1 |
| pEA110, pCDHΔ64 | Methanol | <0.1 |
| pEA110, pCDHΔ64 | Trimethylamine | <0.1 |
| pWM315 | Sodium acetate | <0.1 |
a
β-Galactosidase specific activity is defined as 1 nmol of ONPG-hydrolyzed min−1 μg−1 of protein. The lower detection limit is 0.1 unit. Averages are from three or more replicate cultures.