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. 2005 Aug;71(8):4945–4949. doi: 10.1128/AEM.71.8.4945-4949.2005

FIG. 1.

FIG. 1.

Gel electrophoresis of PCR products from reactions with B. thetaiotaomicron-specific primers B.thetaF and B.thetaR and DNA from human and nonhuman fecal sources. (A) PCR products generated with 1 ng of DNA from each of the human fecal samples, (B) PCR products generated with 1 ng of DNA from each of the sewage samples, and (C) PCR performed with 5 ng of fecal DNA from example subsets of three isolates from each of the 10 sources studied. Sources: sewer, lanes 1, 2, and 3; human, lanes 4, 5, and 6; dairy cattle, lanes 7, 8, and 9; beef cattle, lanes 10, 11, and 12; dog, lanes 13, 14, and 15; chicken, lanes 16, 17, and 18; turkey, lanes 19, 20, and 21; goose, lanes 22, 23, and 24; pig, lanes 25, 26, and 27; horse, lanes 28, 29, and 30. Lanes M have 1,000-bp ladders. Lanes +, positive control with B. thetaiotaomicron DNA. Lanes −, negative control without DNA.