TABLE 2.
Substrate | PH
|
ToMO
|
||||
---|---|---|---|---|---|---|
kcat (s−1)a | Km (μM)b | kcat/Km (s−1 μM−1) | kcat (s−1)a | Km (μM)b | kcat/Km (s−1 μM−1) | |
Phenol | 1.02 | 0.6 | 1.69 | 1 | 2.18 | 0.46 |
p-Cresol | 0.77 | 0.6 | 1.29 | 0.63 | 13.26 | 0.047 |
o-Cresol | 0.946 | 3.13 | 0.3 | 0.9 | 6.0 | 0.15 |
m-Cresol | 0.609 | 1.8 | 0.34 | 0.44 | 9.38 | 0.047 |
2,3-DMP | 0.9425 | 33.87 | 0.028 | 0.2 | 25.7 | 0.008 |
3,4-DMP | 0.771 | 3.37 | 0.228 | 0.38 | 7.7 | 0.049 |
Benzene | 0.092 | 25 | 0.004 | 0.36 | 0.2 | 1.81 |
Toluene | 0.148 | 22 | 0.007 | 0.42 | 3.93 | 0.11 |
o-Xylene | 0.125 | 32.5 | 0.004 | 0.25 | 3.73 | 0.067 |
The errors for kcat values were about 26%. The errors for the kcat values for phenol were about 20%.
The errors for Km values were less than 10%. The error for the Km of ToMO for benzene was about 25%.