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. 2005 Aug;71(8):4736–4743. doi: 10.1128/AEM.71.8.4736-4743.2005

TABLE 2.

Apparent kinetic constants of PH and ToMO

Substrate PH
ToMO
kcat (s−1)a Km (μM)b kcat/Km (s−1 μM−1) kcat (s−1)a Km (μM)b kcat/Km (s−1 μM−1)
Phenol 1.02 0.6 1.69 1 2.18 0.46
p-Cresol 0.77 0.6 1.29 0.63 13.26 0.047
o-Cresol 0.946 3.13 0.3 0.9 6.0 0.15
m-Cresol 0.609 1.8 0.34 0.44 9.38 0.047
2,3-DMP 0.9425 33.87 0.028 0.2 25.7 0.008
3,4-DMP 0.771 3.37 0.228 0.38 7.7 0.049
Benzene 0.092 25 0.004 0.36 0.2 1.81
Toluene 0.148 22 0.007 0.42 3.93 0.11
o-Xylene 0.125 32.5 0.004 0.25 3.73 0.067
a

The errors for kcat values were about 26%. The errors for the kcat values for phenol were about 20%.

b

The errors for Km values were less than 10%. The error for the Km of ToMO for benzene was about 25%.