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. 2005 Aug;71(8):4919–4924. doi: 10.1128/AEM.71.8.4919-4924.2005

FIG. 1.

FIG. 1.

Multiplex PCRs targeting the E. coli O45 wzx and wzy (A) and O55 wzx and wzy (B) genes. (A) Lane M, molecular weight markers; lane 1, E. coli O45 (positive control) (multiplex PCR showing the amplified wzx [255 bp] and wzy [451 bp] genes); lane 2, E. coli K-12 (negative control); lane 3, results from a chicken fecal sample spiked with E. coli O45 at 106 CFU/0.2 g; lane 4, result from a chicken fecal sample spiked with E. coli O45 at 108 CFU/0.2 g. (B) Lane M, molecular weight markers; lane 1, E. coli O55 (positive control) (multiplex PCR showing the amplified wzx [683 bp] and wzy [147 bp] genes); lane 2, E. coli K-12, (negative control); lane 3, results from a chicken fecal sample spiked with E. coli O55 at 106 CFU/0.2 g; lane 4, results from a chicken fecal sample spiked with E. coli O55 at 108 CFU/0.2 g.