TABLE 1.

Genes in the O-antigen gene cluster of E. coli serogroup O45

ORF no.	Proposed gene name	Location (nucleotides)	No. of amino acids in gene product	Putative function	Most significant homolog(s) (accession no.)	% Identity/% similarity
1	rmlB	59-1198	379	dTDP-d-glucose-4,6-dehydratase	RmlB, dTDP-glucose 4,6-dehydratase, Salmonella enterica (AAG09513)	83/87
2	wbhP	1246-2196	316	NAD-dependent epimerase/dehydratase	ORF_12; similar to NAD-dependent epimerase/dehydratase family, Pseudomonas aeruginosa (AAM27579)	42/58
3	wbhQ	2196-3230	344	Glycosyltransferase	Probable N-acetylgalatosaminyl transferase, trsF (S51265), and WbcO, Yersinia enterocolitica (CAA87703)	50/68
4	wbhR	3231-3791	186	Acetyltransferase	Acetyltransferase, Oceanobacillus iheyensis (NP_693813) (BAC14847)	37/58
5	wbhS	3830-5716	628	TrsG protein homolog, role in galactose modification	TrsG protein homolog (T44517), ORF9P (BAA85014), and WbgZ (AAG17416), Plesiomonas shigelloides	61/79
6	rmlC	5797-6336	179	dTDP-4-dehydrorhamnose-3,5-epimerase	Putative dTDP-4-dehydrorhamnose-3,5-epimerase, Aeromonas hydrophila (AAM22546)	66/80
7	wbhT	6333-7151	272	dTDP-glucose-4,6-dehydratase	Putative dTDP-glucose-4,6-dehydratase, Aeromonas hydrophila (AAM22547)	55/69
8	wzx	7144-8403	419	O-antigen flippase	Membrane protein involved in the export of O-antigen and teichoic acid, 12 transmembrane domains, Burkholderia fungorum (ZP_00278649)	25/44
9	wbhU	8387-9352	321	Glycosyltransferase	Putative glycosyltransferase, Pyrococcus furiosus (NP_579088)	29/49
10	wzy	9366-10514	382	O-antigen polymerase	Transmembrane protein, 9 transmembrane domains, Ralstonia solanacearum (NP_519421.1)	27/45
11	wbhV	10507-11043	178	Serine acetyltransferase	Putative acetyltransferase, Clostridium thermocellum (ZP_00313397.1)	37/60
12	wbhW	11045-12055	336	Unknown	Eps11, Streptococcus thermophilus (AAN63793)	30/49
13	rmlA	12102-12974	290	Glucose-1-phosphate thymidylyltransferase	RmlA, Raoultella terrigena (AAQ82933.1)	78/89