TABLE 2.
Oligonucleotide primers and probes
| Target gene | Sequence (5′ to 3′)a | Location in cluster (nucleotides) | Amplicon size (bp) | Accession no. or source |
|---|---|---|---|---|
| Singleplex and multiplex PCR | ||||
| O45 wzx1 | (F) CCG GGT TTC GAT TTG TGA AGG TTG | 7769-8295 | 527 | This study |
| (R) CAC AAC AGC CAC TAC TAG GCA GAA | ||||
| O45 wzx2 | (F) TAT GAC AGG CAC ATG GAT CTG TGG | 7347-7601 | 255 | |
| (R) TTG AGA CGA GCC TGG CTT TGA TAC | ||||
| O45 wzy1 | (F) GAA ATT ATG CCA TCT TGG CGA GCG | 9570-10066 | 497 | This study |
| (R) CAT GTG AAG CCT GAA GGC AAA CTC | ||||
| O45 wzy2 | (F) CTG ATG TCA GGC CTC GTG GAA ATA | 9858-10308 | 451 | |
| (R) ATG TAA CCA CAA TAA GGG AGC CCG | ||||
| O55 wzx1 | (F) AAT GGA ACA TTG CAA CAG CA | 10746-10895 | 150 | AF461121 |
| (R) TGT GGA TTC CAG AAA AGC AA | ||||
| O55 wzx2 | (F) TCT TGT AAC TAA GTG GCC ACA GGC | 10823-11505 | 683 | |
| (R) ATA ACA CCC AAC CTA TAC CTC CCG | ||||
| O55 wzy1 | (F) GTG GTT TTG ACG ACT CGC TT | 9380-9526 | 147 | AF461121 |
| (R) CCA AAA AGC CCT GCA ACT AA | ||||
| Real-time PCR | ||||
| O45 wzx | (F) CGT TGT GCA TGG TGG CAT | 7472-7543 | 72 | This study |
| (R) TGG CCA AAC CAA CTA TGA ACT G | ||||
| O45 wzy | (F) GGT GCT TTG TGA TAA TTC CTG ATG | 9616-9691 | 76 | This study |
| (R) TTA TAG CCG CCC CTA AAT TGC | ||||
| O45 wzx probe | 6-FAM d(ATT TTT TGC TGC AAG TGG GCT GTC CA)BHQ-1 | 7494-7519 | This study | |
| O45 wzy probe | 6-FAM d(TTG CTG CTG GCG GGA TAC CAA TGA T)BHQ-1 | 9643-9667 | This study | |
| O55 wzx | (F) AAT TAA CGA ACA TAA CAC CCA ACC | 11416-11516 | 101 | AF461121 |
| (R) ATA TCT CTT CGT TAC TGT GTG TAT TTC | ||||
| O55 wzy | (F) AGC TTT CCT GGC GGG TTT | 10118-10204 | 87 | AF461121 |
| (R) GCA CCA CGC TAT CTT TTT TCT TAA T | ||||
| O55 wzx probe | 6-FAM d(ACC TCC CGC TAA AAC CCC AAC TCT AGT AG)BHQ-1 | 11461-11489 | AF461121 | |
| O55 wzy probe | 6-FAM d(CCG CGG CGA TAT TGG GTA CTG C)BHQ-1 | 10142-10163 |
a
F, forward; R, reverse.