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. 2005 Aug;71(8):4793–4800. doi: 10.1128/AEM.71.8.4793-4800.2005

TABLE 2.

Fraction of multiplying cells in dilution culture experiments with unamended autoclaved natural seawater as medium

Seawater source (date) Reference Depth (m) Total count (cells ml−6) % Proportion of multiplying (culturability) Method of growth detection
Bay of Banyuls-sur-Mer (2 November 1998) 2 2 0.68 1.6 FCMb
Oregon (May-July)c 7 Surface 1.1-5.6 8.8 DAPIc
Oregon (October-April)a 7 Surface 0.9-1.9 1.2 DAPI
Continental shelf, Japan (12 June 1998) 9 350 0.31 7.7 DAPI
Pacific Ocean (4 June 1998) 9 500 0.55 20 DAPI
Baltic Sea (October 1993) 47 0-30 2.5-3.3 0.1-0.3 DAPI
Baltic Sea (May 1994) 47 0-100 0.7-1.2 7-14 [3H]thymidine
Baltic Sea (May 1994) 47 0-200 0.6-2.7 6-15 [3H]thymidine
North Sea Skagerrak (August 1994) 47 0-25 1.1-1.4 0.5-0.6 [3H]thymidine
North Sea Skagerrak (August 1994) 47 25-340 0.2-0.8 0.2-0.8 [3H]thymidine
Mediterranean Sea (October 1994) 47 Surface 0.5 16 DAPI
a

Samples were collected 1998, 1999, and 2000.

b

Flow cytometry.

c

DAPI staining, fluorescence microscopy.