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Plate assays for the detection of BSH activity. (A) L. acidophilus NCFM; (B) NCFMΔbshA (NCK1618); (C) NCFMΔbshB (NCK1619) on MRS agar containing 0.2% TDCA. In the assays shown, precipitation in the agar is indicative of BSH activity specific for TDCA.
