TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| P. putida KT2440 | mt-2 pWW0 cured | 2 |
| P. putida KT2440 (pWW0) | mt-2; ATCC 33015 | 50 |
| P. putida KT2440/102 | Kmr, P. putida KT2440 with a Km resistance cassette interrupting ORF102 | This study |
| P. putida KT2440/ptsN | Kmr, P. putida KT2440 with a Km resistance cassette interrupting the ptsN gene | This study |
| P. putida KT2440/284 | Kmr, P. putida KT2440 with a Km resistance cassette interrupting ORF284 | This study |
| P. putida KT2440/ptsO | Gmr, P. putida KT2440 with a Gm resistance cassette interrupting the ptsO gene | This study |
| P. putida KT2440/crc | Gmr, P. putida KT2440 with a Gm resistance cassette interrupting the crc gene | This study |
| P. putida KT2440/crp | Kmr, P. putida KT2440 with a Km resistance cassette interrupting the crp gene | This study |
| P. putida KT2440/cyoB | Tcr, P. putida KT2440 with a Tc resistance cassette interrupting the cyoB gene | F. Rojo |
| P. putida KT2440/aer | Kmr, P. putida KT2440 with a Km resistance cassette interrupting the aer gene | V. Shingler |
| P. putida KT2440/relA | Kmr, P. putida KT2440 with a Km resistance cassette interrupting the relA gene | V. Shingler |
| P. putida KT2440/spoT-relA | Kmr, Gmr, P. putida KT2440/relA with a Gm resistance cassette interrupting the spoT gene | V. Shingler |
| P. putida KT2440/crc-ptsN | Gmr, Kmr, P. putida KT2440/ptsN with a Gm resistance cassette interrupting the crc gene | This study |
| E. coli DH5α | F− Φ80d lacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 R− M+supE44 thi1 gyrA relA1 | 21 |
| E. coli HB101 | Smr, hsd R− M+ pro leu thi recA | 43 |
| Plasmids | ||
| pGEM-T | PCR product cloning vector | Promega |
| pKNG101 | Smr, sucrose sensitive, ori RK2 | 26 |
| pKNG102 | Smr, Kmr (with a Km resistance cassette inserted in ORF102) | 9 |
| pKNG154 | Smr, Kmr (with a Km resistance cassette inserted in the ptsN gene) | 9 |
| pKNG284 | Smr, Kmr (with a Km resistance cassette inserted in ORF284) | 9 |
| pKNGptsO | Smr, Gmr (with a Gm resistance cassette inserted in the ptsO gene) | This study |
| pKNGcrc | Smr, Gmr (with a Gm resistance cassette inserted in the crc gene) | This study |
| pKNGcrp | Smr, Gmr (with a Km resistance cassette inserted in the crp gene) | This study |
| pRK600 | Cmr, oriColE1, mobRK2, traRK2 | 27 |
| pS10 | IncP1, Smr, xylR, transcriptional Pu::lacZ::tet fusion | This study |
| pWW0 | IncP9, mob+, tra+, 3MB+ | 50 |