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GC-MS analysis of P. putida CA-3 mini-Tn5 mutants
| P. putida CA-3 | Growth substrate (mM) | PHA (% CDW) |
|---|---|---|
| Wild type | PA (10) | 23.2 |
| E28G12a | PA (10) | 0 |
| E22B11a | PA (10) | 0 |
E28G12 and E22B11 are both PhaC1 synthase-negative mutants. Cells were grown on PA for 48 h on E2 medium-8 mM NH4+ before assessment for PHA accumulation.
