TABLE 2.
Comparison of the enzymatic activity of Paecilomyces variotii biomass produced in liquid culture and in STBE
Experimental system | Oxygenase sp act (nmol O2/min/mg biomass)a | Dehydrogenase sp act (nmol NADH/min/mg protein)b |
---|---|---|
Biomass from liquid culture | 4.7 | ND |
Cell extract | ND | 16.2 (NAD+) |
4.6 (NADP+) | ||
Biomass from STBE | 6.3 | ND |
Homogenized | 11.9 | ND |
Cell extract | ND | 36.6 (NAD+) |
0.2 (NADP+) |
Measured with a toluene concentration of 87 mg/liter.
Benzyl alcohol dehydrogenase activity was measured in cell extracts obtained from the liquid culture and the STBE. ND, not determined.