FIG. 2.

FACSVantage dual-parameter dot plots of a fecal sample from adult A stained with SYTO BC and PI. A region (R1) was defined around the whole cell population in the dot plot on the left. In the dot plot on the right, regions were set around target populations as follows: SYTO BC-stained cells (R2), double-stained cells (R3), and PI-stained cells. A gate was defined whereby any particle present within R1 and R2 was sorted as a live cell, any particle present within R1 and R3 was sorted as an injured cell, and any particle present within R1 and R4 was sorted as a dead cell. Sorted cells were recovered in separate sterile tubes for a range of 1 × 10⁶ to 5 × 10⁶ cells.