TABLE 2.
Viability assessment of fecal microbiota using FCM and membrane integrity probes (PI and SYTO BC)
| Adult | Total counta | Eub338 countb | Viability count (%)c
|
||
|---|---|---|---|---|---|
| Viable cells | Dead cells | Injured cells | |||
| A | 1.68 × 1011 | 1.24 × 1011 | 42.9 | 31.3 | 25.8 |
| B | 6.06 × 1010 | 3.94 × 1010 | 51.7 | 28.8 | 19.5 |
| C | 6.88 × 1010 | 5.44 × 1010 | 59.4 | 33.5 | 7.1 |
| D | 4.64 × 1010 | 2.97 × 1010 | 41.8 | 33.2 | 25.0 |
| Mean (SD) | 8.60 × 1010 (5.55 × 1010) | 6.15 × 1010 (4.20 × 1010) | 49.0 ± 8.2 | 31.7 ± 2.2 | 19.4 ± 8.7 |
a
Number of fecal cells/gram (wet weight) of feces determined by SYTO BC staining.
b
Number of cells hybridized by the bacterial probe Eub338 labeled with Cy5.
c
Percentages of viable, dead, and injured cells as monitored by the combination of the fluorescent dyes PI and SYTO BC.