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Appearance of WT (PAO1 lasB::gfp) and ST (MJK3) colony morphology variants on solid medium and in liquid culture. When grown on solid medium, the wild-type strain formed large flat colonies (a), whereas ST formed small, wrinkled, sticky colonies (b). In shaken liquid culture, WT yielded a homogeneous culture (c), but ST tended to grow as a clump (d). As shown by SEM, a WT tube biofilm was flat (e), and an ST tube biofilm had an irregular, wrinkled surface (f). Magnification, ×1,000.
