FIG. 1.

Incidence of secondary symbionts in aphids and cell cultures. (a) T type. (b) U type. The aphid source material was M. rosae line ACD03/01 (lane 2), A. pisum line JF99/14 (lane 4), and A. fabae line CRT01/43 (lane 6). C6/36 cells were infected with bacterial preparations from M. rosae (lane 3), A. pisum (lane 5), and A. fabae (lane 7). Lanes 8 and 9 contained positive controls for T type and U type, respectively, and lane 10 contained the template-free negative control. The molecular ladder (lanes 1 and 11) was the BIOTC medium ladder (Geneflow, Fradley, United Kingdom). The bands were at (in descending order) bp 2686, 2000, 1500, 1200, 1000, 900, 800, 700, 500, 400, 300, 200, and 100. The analysis was conducted on a 1% agarose gel.