TABLE 2.
Esterification activities of mutated ROLs displayed on the yeast cell surface in heptane containing 0.2% H2O
| Clone | Conversion rates (%) with the following fatty acidsa:
|
|||
|---|---|---|---|---|
| C10:0 | C14:0 | C18:0 | C22:0 | |
| Wild typeb | 11 ± 1c | 17 ± 1 | 12 ± 1 | 10 ± 1 |
| E5 (K51E) | 38 ± 3 | 54 ± 5 | 45 ± 4 | 38 ± 3 |
| E8 (G3A, K51E) | 47 ± 4 | 59 ± 6 | 51 ± 5 | 46 ± 4 |
| E10 (S267N) | 41 ± 4 | 58 ± 5 | 48 ± 4 | 41 ± 4 |
| E17 (A24R) | 59 ± 6 | 75 ± 7 | 67 ± 6 | 60 ± 6 |
| E31 (T241S) | 46 ± 4 | 61 ± 6 | 53 ± 5 | 45 ± 4 |
a
C10:0, capric acid; C14:0, myrisric acid; C18:0, stearic acid; C22:0, behenic acid.
b
Cells (3.0 mg [dry weight]) were added to 1-ml reaction mixtures. The wild type was the wild-type ROL-displaying yeast.
c
Conversion rates after 3 h. The data are means ± standard errors based on three independent measurements.