FIG. 5.

Selective removal of Streptomyces DNA in an environmental DNA sample (compost) spiked with different levels of S. coelicor DNA. All samples contained 30 ng of environmental DNA with 0 ng (lanes 1E and 1N), 33 ng (lane 2E), 66 ng (lane 3E), 99 ng (lane 4E), 132 ng (lane 5E), or 165 ng (lanes 6E and 6N) of amended Streptomyces DNA. Control reaction mixtures to which no restriction enzyme was added were also used (lanes 1N and 6N). After the SuPER reaction, all samples were subject to PCR-DGGE analyses with general bacterial primers. The compost bacterial population profile was restored in those samples spiked with Streptomyces DNA and subjected to the active (enzyme added) SuPER reaction.