TABLE 1.

Primer sequences

Primer	Sequence (5′→3′)	SSU rRNA gene location (positions)b	Target population	Reference or source
SuPER reaction
Q491-F	GGG GAA TAA GCA TCG GCT AAC TCT G	491-515	Plastids	This study
Q491-R	CAG AGT TAG CCG ATG CTT ATT CCC C	515-491	Plastids	This study
S661-F	GTA GGG GAG ATC GGA ATT	661-678	Streptomyces	6
S661-R	AAT TCC GAT CTC CCC TAC	678-661	Streptomyces	6
Bacterial 16S rRNA gene amplification
341-Fa	CCT ACG GGA GGC AGC AG	341-357	Bacteria	9
907-R	CCG TCA ATT CMT TTG AGT TT	926-907	Bacteria	9

^aContains the following 40-base GC clamp attached to the 5′ end to enhance separation in DGGE analyses: CGC CCG CCG CGC CCC GCG CCC GTC CCG CCG CCC CCG CCC G.

^bSSU, small subunit.