TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmida | Relevant characteristic(s)b | Reference and/or source |
|---|---|---|
| Strains | ||
| Brevundimonas sp. strain SD212 | 2-Hydroxyastaxanthin-producing bacterium | 55, MBIC03018 |
| Escherichia coli XL1-Blue MR | Host strain of cosmid vector SuperCos 1 | Stratagene |
| E. coli DH5α | Host strain for DNA manipulation | Toyobo |
| E. coli JM109 | Host strain for heterologous expression of crt genes | Toyobo |
| Plasmids | ||
| pACCAR25ΔcrtEc | Cmr, plasmid carrying crtB, crtI, crtY, crtZ, and crtX | 41 |
| pACCAR25ΔcrtBc | Cmr, plasmid carrying crtE, crtI, crtY, crtZ, and crtX | 9 |
| pACCRT-EBc | Cmr, plasmid carrying crtE and crtB | 31 |
| pACCRT-EIBc | Cmr, plasmid carrying crtE, crtB, and crtI | 31 |
| pACCAR16ΔcrtXc | Cmr, plasmid carrying crtE, crtB, crtI, and crtY | 31 |
| pACCAR25ΔcrtXc | Cmr, plasmid carrying crtE, crtB, crtI, crtY, and crtZ | 31 |
| pAC-Canthad | Cmr, plasmid carrying crtE, crtB, crtI, crtY, and crtW | This study |
| pAC-Astad | Cmr, plasmid carrying crtE, crB, crtI, crtY, crtZ, and crtW | This study |
| SuperCos 1 | Apr, cosmid vector | Stratagene |
| pBluescript II KS(−) | Apr, cloning vector for E. coli | Toyobo |
| pGEM-T Easy | Apr, cloning vector for E. coli | Promega |
| pUC18 | Apr, cloning vector for E. coli | Toyobo |
| pCos5-2 | Apr, 47-kb fragment (partial Sau3AI digest) of strain SD212 inserted into the BamHI site of SuperCos 1 | This study |
| pCRTI-SD212 | Apr, 1.1-kb fragment containing crtI amplified by PCR inserted into pGEM-T Easy | This study |
| p5Bre2-15 | Apr, 12-kb EcoRI fragment derived from pCos5-2 inserted into pBluescript II KS(−) | This study |
| pUCBre-O11 | Apr, 0.77-kb fragment containing ORF11 amplified by PCR derived from p5Bre2-15 inserted into pUC18 | This study |
a
Plasmids for the expression of individual genes or ORFs from Brevundimonas sp. strain SD212 in E. coli are not included in this table, except for pUCBre-O11.
b
Apr, ampicillin resistance; Cmr, chloramphenicol resistance.
c
The plasmids contain the crt genes from Pantoea ananatis (Erwinia uredovora 20D3).
d
The plasmids contain the crtW gene from Paracoccus sp. strain N81106 in addition to the crt genes from P. ananatis.