FIG. 1.

(A) Genomic organization of the yyc operon in B. subtilis and the corresponding region of the L. monocytogenes EGDe genome. (B) Kyte and Doolittle hydrophobicity plots of HtrA in L. monocytogenes EGDe (gray line indicates putative leader peptide). (C) The frameshift mutation in the potential signal sequence of HtrA is represented by a dash. (D) Transcriptional analysis of lmo0291 and lmo0293. Total RNA was isolated from exponential-phase cultures of EGDe and the htrA mutant grown in BHI at 37°C. RNA was converted to cDNA, and PCRs were performed with lmo0291 and lmo0293 specific primers.