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(A) Growth of wild-type EGDe (•), revertant (○), htrA mutant (▾), and HtrASS* (▿) under sublethal heat conditions (44°C). Error bars represent standard deviations of triplicate experiments. (B) Transcriptional analysis of htrA by RT-PCR. Total RNA was isolated from exponential-phase cultures of EGDe grown in BHI at 37°C (Control) and exposed to 45°C for 30 min (Adapted). RNA was converted to cDNA and PCRs were performed with htrA, groEL, and dnaK specific primers.
