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. 2005 Aug;71(8):4241–4247. doi: 10.1128/AEM.71.8.4241-4247.2005

FIG. 3.

FIG. 3.

(A) Growth of wild-type EGDe (•), revertant (○), htrA mutant (▾), and HtrASS* (▿) under sublethal acid conditions (pH 5, HCl). Error bars represent standard deviations of triplicate experiments. (B) Transcriptional analysis of htrA by RT-PCR. Total RNA was isolated from exponential-phase cultures of EGDe grown in pH 7 BHI (Control) and exposed to pH 5 for 30 min (Adapted). RNA was converted to cDNA, and PCRs were performed with htrA specific primers.