FIG. 4.

(A) Growth of wild-type EGDe (•), revertant (○), htrA mutant (▾), and HtrASS* (▿) under sublethal penicillin G conditions (87.5 ng/ml). Error bars represent standard deviations of triplicate experiments. (B and C) Transcriptional analysis of htrA by RT-PCR. (B) Total RNA was isolated from exponential-phase cultures of EGDe growing in BHI (Control) and exposed to penicillin G at 87.5 ng/ml for 30 min (Adapted). (C) Total RNA was extracted from stationary-phase cultures of LO28 wild-type and ΔlisK strains. RNA was converted to cDNA, and PCRs were performed with htrA specific primers.