TABLE 2.
Primer | Sequence (5′-3′) |
---|---|
HtrAF | GGTCTAGACGCGCTGGTACAACTGGa |
HtrAR | TCGGATCCGTCGCCATTTGTATCAACa,c |
HtrA-out | ACCGGAGAGCGCGCAACCAGc |
HtrASSF | AACGGATCCTGGTTATGTAAGa |
HtrASSR | ACGGTACCCGTTACCACTTCa |
HtrASSQ1F | CACCGTTTTTTTAAAGCTTTATTCATC GTTTTTTCATATTTGGGGb |
HtrASSQ1R | CCCCAAATATGAAAAAACGATGAATA AAGCTTTAAAAAAACGGTb |
DnaKF | GCTGGTCTTGAAGTAGAACc |
DnaKR | GTTCATCAAATTTAGCACGAGTc |
GroELF | GTAGTAGCCGTGAAAGCc |
GroELR | GTAGAGCGGAACGTGTTACc |
0291F | GATTGTGCTTTGAGTGGAAAGc |
0291R | CCCCCAACATCAATACCTTCc |
0293F | GTTGAAGTTCCGGATGAGAAAc |
0293R | CAACCTCATCAACTGGTGCc |
Restriction sites incorporated into primer sequences are underlined.
Frameshift mutation incorporated into primer sequence are underlined.
Primers used for transcriptional analysis.