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. 2005 Aug;71(8):4241–4247. doi: 10.1128/AEM.71.8.4241-4247.2005

TABLE 2.

Oligonucleotide primers

Primer Sequence (5′-3′)
HtrAF GGTCTAGACGCGCTGGTACAACTGGa
HtrAR TCGGATCCGTCGCCATTTGTATCAACa,c
HtrA-out ACCGGAGAGCGCGCAACCAGc
HtrASSF AACGGATCCTGGTTATGTAAGa
HtrASSR ACGGTACCCGTTACCACTTCa
HtrASSQ1F CACCGTTTTTTTAAAGCTTTATTCATC GTTTTTTCATATTTGGGGb
HtrASSQ1R CCCCAAATATGAAAAAACGATGAATA AAGCTTTAAAAAAACGGTb
DnaKF GCTGGTCTTGAAGTAGAACc
DnaKR GTTCATCAAATTTAGCACGAGTc
GroELF GTAGTAGCCGTGAAAGCc
GroELR GTAGAGCGGAACGTGTTACc
0291F GATTGTGCTTTGAGTGGAAAGc
0291R CCCCCAACATCAATACCTTCc
0293F GTTGAAGTTCCGGATGAGAAAc
0293R CAACCTCATCAACTGGTGCc
a

Restriction sites incorporated into primer sequences are underlined.

b

Frameshift mutation incorporated into primer sequence are underlined.

c

Primers used for transcriptional analysis.