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. 2005 Aug;71(8):4352–4358. doi: 10.1128/AEM.71.8.4352-4358.2005

FIG. 1.

FIG. 1.

Determination of molecular mass of purified P. horikoshii NAD kinase. (A) SDS-PAGE of recombinant P. horikoshii NAD kinase. Left lane, protein markers (New England Biolabs); right lane, purified P. horikoshii NAD kinase. (B) Molecular mass determination of native P. horikoshii NAD kinase by gel filtration chromatography. The molecular mass was plotted versus the elution volume/void volume. The void volume was determined with blue dextran 2000 (2,000 kDa). The elution position of the purified enzyme is indicated by a filled circle.