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Initial-velocity analyses of NAD kinase. (A) Double-reciprocal plot of enzyme activity against ATP concentration in the presence of 5.0 mM NAD; (B) double-reciprocal plot of enzyme activity against NAD concentration in the presence of 5.0 mM ATP; (C) double-reciprocal plot of enzyme activity against NAD concentration in the presence of 2.0 mg poly(p)27; (D) dependence of the initial velocity on poly(P)27 concentration.
