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Summary of purification of P. horikoshii NAD kinase from recombinant E. coli cells
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 557 | 501 | 0.90 | 100 | 1.0 |
| Heat treatment at 90°C for 20 min | 59.9 | 421 | 7.0 | 84 | 7.8 |
| HiTrap chromatography | 22.5 | 174 | 7.6 | 35 | 8.4 |
